Besides, this protocol also provides detailed procedures on the (i) G. This allows the continuous supply of healthy G. mellonella without requiring special instruments and specialized training. Here, we present a protocol for simple rearing and maintenance of G. They act as suitable preliminary infection models to study host-pathogen interactions due to their advantages, such as the ability to survive at 37☌ mimicking human body temperature, their immune system shares similarities with mammalian immune systems, and their short life cycle allowing large-scale studies. Galleria mellonella larvae have been increasingly used in research, including microbial infection studies.
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